Author : Vijay Gadkar, Alok Adholeya.

Address :

Abstract : Intraradical sporulation of AM Gigaspora margarita in long-term axenic cultivation in Ri T-DNA carrot root.

Journal : Mycological Research,

Volume No. :

Publish Year : 2000

Page No. : 716-721

Main Data : Arbuscular mycorrzhial (AM) fungi are obgligate biotrophic organisms. Root organ culture(ROC) can be used to grow these fungi under in vitro conditions. The ROC technique was used with Giaspora margarita and Ri T-DNA transformed carrot roots to examine the fungal growth and physiology under long term axenic symbiosis. The fungus formed spores inside the host roots under in vitro conditions. Sporulation was a temporal phenomenon found in dual cultures more than 18-20 mo old. The spores formed intraradically were 10-15% of the total spores formed in a single culture. These intraradical spores were analysed for their morphology and DNA polymorphism pattern with spores which has formed conventionally in the medium. Both anslyses showed no detectable cariation. This is the first report of spore formation by Gigaspora inside the roots of a host.